Exposure of actin thiols by the removal of tightly held calcium ions.

نویسندگان

  • K Konno
  • M F Morales
چکیده

The removal of bound metal ions from G-actin uncovered two thiols, Cys-10 and Cys-257. The uncovering of these thiols requires a free calcium concentration lower than 10 nM. Therefore, participation of one or both thiols in Ca2+ binding is suggested. Actin labeled with N-(5-fluoresceinyl)maleimide in the absence of calcium moves as a doublet in NaDodSO4/PAGE. It is suggested that two conformers are induced by metal removal and labeling.

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عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 82 23  شماره 

صفحات  -

تاریخ انتشار 1985